Implications of the Actin Cytoskeleton on the Multi-Step Process of [ PSI+] Prion Formation

Authors

Jane E. Dorweiler, Marquette UniversityFollow
Douglas R. Lyke, Marquette UniversityFollow
Nathan P. LeMoine, Marquette UniversityFollow
Samantha Guereca, Marquette UniversityFollow
Hannah E. Buchholz, Marquette UniversityFollow
Emily R. Legan, Marquette UniversityFollow
Claire M. Radtke, Marquette UniversityFollow
Anita L. Manogaran, Marquette UniversityFollow

Document Type

Article

Publication Date

7-2022

Publisher

MDPI

Source Publication

Viruses

Source ISSN

1999-4915

Original Item ID

DOI: 10.3390/v14071581

Abstract

Yeast prions are self-perpetuating misfolded proteins that are infectious. In yeast, [PSI⁺] is the prion form of the Sup35 protein. While the study of [PSI⁺] has revealed important cellular mechanisms that contribute to prion propagation, the underlying cellular factors that influence prion formation are not well understood. Prion formation has been described as a multi-step process involving both the initial nucleation and growth of aggregates, followed by the subsequent transmission of prion particles to daughter cells. Prior evidence suggests that actin plays a role in this multi-step process, but actin’s precise role is unclear. Here, we investigate how actin influences the cell’s ability to manage newly formed visible aggregates and how actin influences the transmission of newly formed aggregates to future generations. At early steps, using 3D time-lapse microscopy, several actin mutants, and Markov modeling, we find that the movement of newly formed aggregates is random and actin independent. At later steps, our prion induction studies provide evidence that the transmission of newly formed prion particles to daughter cells is limited by the actin cytoskeletal network. We suspect that this limitation is because actin is used to possibly retain prion particles in the mother cell.

Comments

Published version. Viruses, Vol. 14, No. 1 (July 2022): 1581. DOI. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Dorweiler, Jane E.; Lyke, Douglas R.; LeMoine, Nathan P.; Guereca, Samantha; Buchholz, Hannah E.; Legan, Emily R.; Radtke, Claire M.; and Manogaran, Anita L., "Implications of the Actin Cytoskeleton on the Multi-Step Process of [ PSI+] Prion Formation" (2022). Biological Sciences Faculty Research and Publications. 888.
https://epublications.marquette.edu/bio_fac/888