Genome-Wide Identification of the A20/AN1 Zinc Finger Proteon Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance

Authors

Hao Xie, Northeast Forestry University
Qiangqiang Yang, Chinese Academy of Agricultural Studies
Xiaoxiao Wang, Chinese Academy of Agricultural Studies
Michael Schläppi, Marquette UniversityFollow
Hui Yan, Chinese Academy of Agricultural Studies
Meng Kou, Chinese Academy of Agricultural Studies
Wei Tang, Chinese Academy of Agricultural Studies
Xin Wang, Chinese Academy of Agricultural Studies
Yungang Zhang, Chinese Academy of Agricultural Studies
Qiang Li, Chinese Academy of Agricultural Studies
Shaojun Dai, Shanghai Normal University
Yaju Liu, Chinese Academy of Agricultural Studies

Document Type

Article

Publication Date

10-2022

Publisher

MDPI

Source Publication

International Journal of Molecular Sciences

Source ISSN

1422-0067

Original Item ID

DOI: 10.3390/ijms231911551

Abstract

Stress-associated protein (SAP) genes—encoding A20/AN1 zinc-finger domain-containing proteins—play pivotal roles in regulating stress responses, growth, and development in plants. They are considered suitable candidates to improve abiotic stress tolerance in plants. However, the SAP gene family in sweet potato (Ipomoea batatas) and its relatives is yet to be investigated. In this study, 20 SAPs in sweet potato, and 23 and 26 SAPs in its wild diploid relatives Ipomoea triloba and Ipomoea trifida were identified. The chromosome locations, gene structures, protein physiological properties, conserved domains, and phylogenetic relationships of these SAPs were analyzed systematically. Binding motif analysis of IbSAPs indicated that hormone and stress responsive cis-acting elements were distributed in their promoters. RT-qPCR or RNA-seq data revealed that the expression patterns of IbSAP, ItbSAP, and ItfSAP genes varied in different organs and responded to salinity, drought, or ABA (abscisic acid) treatments differently. Moreover, we found that IbSAP16 driven by the 35 S promoter conferred salinity tolerance in transgenic Arabidopsis. These results provided a genome-wide characterization of SAP genes in sweet potato and its two relatives and suggested that IbSAP16 is involved in salinity stress responses. Our research laid the groundwork for studying SAP-mediated stress response mechanisms in sweet potato.

Comments

Published version. International Journal of Molecular Sciences, Vol. 23, No. 19 (September 2022). DOI. © 2022 MDPI. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Xie, Hao; Yang, Qiangqiang; Wang, Xiaoxiao; Schläppi, Michael; Yan, Hui; Kou, Meng; Tang, Wei; Wang, Xin; Zhang, Yungang; Li, Qiang; Dai, Shaojun; and Liu, Yaju, "Genome-Wide Identification of the A20/AN1 Zinc Finger Proteon Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance" (2022). Biological Sciences Faculty Research and Publications. 933.
https://epublications.marquette.edu/bio_fac/933