Navigating Rice Seedling Cold Resilience: QTL Mapping in Two Inbred Line Populations and the Search for Genes

Authors

Michael Schläppi, Marquette UniversityFollow
Avery R. Jessel, Marquette University
Aaron K. Jackson, Dale Bumpers National Rice Research Center
Huy Phan, Marquette University
Melissa H. Jia, Dale Bumpers National Rice Research Center
Jeremy D. Edwards, Dale Bumpers National Rice Research Center
Georgia C. Eizenga, Dale Bumpers National Rice Research Center

Document Type

Article

Publication Date

2023

Publisher

Frontiers Media S.A.

Source Publication

Frontiers in Plant Science

Source ISSN

1664-462X

Original Item ID

DOI: 10.3389/fpls.2023.1303651

Abstract

Due to global climate change resulting in extreme temperature fluctuations, it becomes increasingly necessary to explore the natural genetic variation in model crops such as rice to facilitate the breeding of climate-resilient cultivars. To uncover genomic regions in rice involved in managing cold stress tolerance responses and to identify associated cold tolerance genes, two inbred line populations developed from crosses between cold-tolerant and cold-sensitive parents were used for quantitative trait locus (QTL) mapping of two traits: degree of membrane damage after 1 week of cold exposure quantified as percent electrolyte leakage (EL) and percent low-temperature seedling survivability (LTSS) after 1 week of recovery growth. This revealed four EL QTL and 12 LTSS QTL, all overlapping with larger QTL regions previously uncovered by genome-wide association study (GWAS) mapping approaches. Within the QTL regions, 25 cold-tolerant candidate genes were identified based on genomic differences between the cold-tolerant and cold-sensitive parents. Of those genes, 20% coded for receptor-like kinases potentially involved in signal transduction of cold tolerance responses; 16% coded for transcription factors or factors potentially involved in regulating cold tolerance response effector genes; and 64% coded for protein chaperons or enzymes potentially serving as cold tolerance effector proteins. Most of the 25 genes were cold temperature regulated and had deleterious nucleotide variants in the cold-sensitive parent, which might contribute to its cold-sensitive phenotype.

Comments

Published version. Frontiers in Plant Science, Vol. 14 (2023). DOI. © 2023 Frontiers Media S.A. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Schläppi, Michael; Jessel, Avery R.; Jackson, Aaron K.; Phan, Huy; Jia, Melissa H.; Edwards, Jeremy D.; and Eizenga, Georgia C., "Navigating Rice Seedling Cold Resilience: QTL Mapping in Two Inbred Line Populations and the Search for Genes" (2023). Biological Sciences Faculty Research and Publications. 963.
https://epublications.marquette.edu/bio_fac/963