Diagnosis of Schistosoma mansoni without the Stool: Comparison of Three Diagnostic Tests to Detect Schiostosoma mansoni Infection from Filtered Urine in Zambia

Authors

Nilanjan Lodh, Marquette UniversityFollow
James C. L. Mwansa, Johns Hopkins UniversityFollow
Mabel M. Mutengo, Johns Hopkins University
Clive J. Shiff, Johns Hopkins UniversityFollow

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

2013

Publisher

American Society of Tropical Medicine and Hygiene

Source Publication

American Journal of Tropical Medicine and Hygiene

Source ISSN

1476-1645

Original Item ID

DOI: 10.4269/ajtmh.13-0104

Abstract

Diagnosis for intestinal Schistosoma mansoni lacks sensitivity and is arduous to conduct. The standard diagnostic tests, Kato-Katz (KK) and circulating cathodic antigen (CCA) both lack sensitivity and with KK, require obtaining, transporting, and examining fresh stool. We compared diagnostic efficacy of KK, CCA, and polymerase chain reaction (PCR) to detect S. mansoni infection (species-specific DNA) from 89 filtered urine samples collected in Zambia. The PCR was the strongest indicator of positive cases with sensitivity and specificity of 100% in comparison to CCA (67% and 60%) and KK (50% and 100%). High positive and negative predictive values (100%) were also indicative of robustness of PCR. The same pattern was observed when stratified for sex and age group-specific analysis. Diagnosis of S. mansoni from filtered urine samples by PCR is an effective means to detect low intensity infection and would enhance the effectiveness of surveillance and control programs of schistosomiasis.

Comments

Published version. American Journal of Tropical Medicine and Hygiene, Vol. 89, No. 1 (2013): 46-50. DOI. © 2013 American Society of Tropical Medicine and Hygiene. Used with permission.

Nilanjan Lodh was affiliated with Johns Hopkins University at the time of publication.

Recommended Citation

Lodh, Nilanjan; Mwansa, James C. L.; Mutengo, Mabel M.; and Shiff, Clive J., "Diagnosis of Schistosoma mansoni without the Stool: Comparison of Three Diagnostic Tests to Detect Schiostosoma mansoni Infection from Filtered Urine in Zambia" (2013). Clinical Lab Sciences Faculty Research and Publications. 12.
https://epublications.marquette.edu/clinical_lab_fac/12