Mycoplasmoides genitalium Nucleic Acid Semi-quantitation and Molecular Macrolide Resistance Detection via Automated Assays: Gender and Specimen Source Considerations
Document Type
Article
Publication Date
6-2024
Publisher
American Society for Microbiology
Source Publication
Journal of Clinical Microbiology
Source ISSN
0095-1137
Original Item ID
DOI: 10.1128/jcm.00485-24
Abstract
A laboratory-developed test (LDT) using analyte-specific reagents has been optimized on a commercial platform to detect macrolide resistance-associated mutations (MRM) in 23S rRNA from Mycoplasmoides genitalium from primary clinical specimens. In this study, MRM-LDT was applied to a multi-specimen source study set. One thousand four hundred ninety-five primary specimens testing positive for M. genitalium by commercial transcription-mediated amplification (TMA) were initially titered by the TMA assay using serial 10-fold dilutions to semi-quantitate target nucleic acid burden. Primary specimens were then processed for MRM detection using the MRM-LDT. Findings were stratified by gender and specimen source. The mean log10 target nucleic acid titer of a TMA-positive specimen was 3.51 (median 3; range 0–10). Male specimens (n = 1145) demonstrated a mean log10 M. genitalium TMA titer of 3.67; that value observed in 350 female specimens was 2.98 (P < 0.0001). The MRM-LDT detection rate (88.7%) from specimens with log10 M. genitalium TMA titers ≥ 4 was increased over specimens with log10 titers ≤ 1 (4.5%; P < 0.0002). In females, MRM-LDT was positive from 51.3% of vaginal swab and 34.7% of urine specimens (P = 0.01). In males, MRM-LDT was positive from 65.0% of rectal swab and 55.7% of urine specimens (P = 0.002). Differences were also observed in log10 M. genitalium TMA titers as a function of specimen source. M. genitalium macrolide resistance rates among multiple specimen sources, as determined by MRM-LDT, are high in the United States and can be consistent with target nucleic acid burden within the primary specimen. Caveats experienced within subgroupings support MRM reflex testing on primary M. genitalium-positive specimens.
Recommended Citation
Munson, Erik; Moore, Josephine; Kreuger, Trinity; Zapp, Amanda; Lavey, Stephen C.; Munson, Kimber L.; Stafford, Irene A.; and Mustanksi, Brian, "Mycoplasmoides genitalium Nucleic Acid Semi-quantitation and Molecular Macrolide Resistance Detection via Automated Assays: Gender and Specimen Source Considerations" (2024). Clinical Lab Sciences Faculty Research and Publications. 74.
https://epublications.marquette.edu/clinical_lab_fac/74
Comments
Journal of Clinical Microbiology, Vol. 62, No. 6 (2024). DOI.