Document Type
Article
Publication Date
2-2026
Publisher
Wiley
Source Publication
European Journal of Oral Sciences
Source ISSN
0909-8836
Abstract
This study aims to investigate the trans-dentinal and direct cell viability of ammonia- and water-based silver fluoride treatments. Deep dentin discs were prepared, balanced for permeability and autoclaved. Three-dimensional cultures of odontoblast-like cells were transferred to the pulpal aspect of the dentin slices inside perfusion split chambers designed for dentin-barrier cytotoxicity test, following ISO 7405. An experimental resin-based glass ionomer cement and a polyvinylsiloxane impression material served as positive and negative controls, respectively. The experimental treatments included: (i) ammonia-based silver fluoride = SDF, (ii) SDF + potassium iodide = KI, (iii) water-based silver fluoride = SF, and (iv) SF + KI. Treatments were applied to the occlusal surface of dentin discs and cell viability (%) was assessed after 24 h using the methylthiazolium (MTT) assay. The cytotoxicity of dilutions (10−3, 10−4, and 10−5) were evaluated with direct exposure, using the same cell line following ISO 10993-5. SF treatment revealed the highest cell viability among the treatment groups for the dentin-barrier test. In direct cytotoxicity test, SDF and SF treatments exhibited no cytotoxicity at 10−4 and 10−5 dilutions. The addition of KI increased cytotoxicity. Ammonia- and water-based silver fluoride treatments, particularly in deep cavities, should be applied with caution.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Uctasli, Merve; Seseogullari-Dirihan, Roda; Mutluay, Mustafa Murat; and Tezvergil-Mutluay, Arzu, "Cytotoxicity Of Ammonia- And Water-Based Silver Fluoride Treatments" (2026). School of Dentistry Faculty Research and Publications. 594.
https://epublications.marquette.edu/dentistry_fac/594
Comments
Published version. European Journal of Oral Sciences, Vol. 134, No. 1 (February 2026). DOI. © 2025 The Author(s). European Journal of Oral Sciences published by John Wiley & Sons Ltd on behalf of Scandinavian Division of the International Association for Dental Research.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.