Date of Award


Document Type

Dissertation - Restricted

Degree Name

Doctor of Philosophy (PhD)



First Advisor

Daniel Haworth

Second Advisor

Bruce Pollard

Third Advisor

Karen Fallgatter

Fourth Advisor

Michael D. Ryan

Fifth Advisor

David Schrader


A continuum source atomic fluorescence spectrometer (CSAFS) has been constructed for use in high performance liquid chromatography (HPLC). The design is described in detail, and analytical figures of merit such as reproducibility and limits of detection are presented for the spectrometer.

The CSAF spectrometer was interfaced to a high performance liquid chromatograph for metal specific detection. Details of this interface are presented, as well as performance characteristics of the interfaced system. The HPLC-CSAFS system was used to speciate cadmium ion from cadmium complexed to soil fulvic a new method for determining the stability constant of that reaction based on this separation is presented.

The peak shapes of the system are described in terms of two models. These models examine the physical and chemical processes of the detector and how these processes affect the peak shape. Computer simulations are used to fit experimental data to the models.

A theoretical framework is described which extends present signal-to-noise theory for static systems to dynamic, time-dependent methods. These equations define dynamic figures of merit, particularly signal-to-noise ratio and limit of detection, in terms of their static system counterparts. The postulations are verified by experimentation on the HPLC-CSAFS system and by computer simulation of noise processes.



Restricted Access Item

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