Date of Award

4-1963

Degree Type

Master's Essay

Degree Name

Master of Science (MS)

Department

Medical

First Advisor

Paul M. Roll

Second Advisor

Michael Laskowski

Third Advisor

Clifford R. Haymaker

Abstract

Evidence was presented in 1949 by Bendich, Getler, and Brown (2), that the free pyrimidine base, cytosine, was not incorporated into the nucleotides of deoxyribonucleic acid (DNA). Further studies by Hammarsten, Reichard, and Saluste (9) with cytidine and those by Roll, Weinfeld, and Carroll (26) with cytidylic acid, showed that the nucleoside and nucleotide derivatives were well incorporated into DNA. These experiments indicated that the conversion of the riboside (or ribotide) to the deoxyribose form had occurred with the nucleosidic linkage intact. This theory was further substantiated by Rose and Schweigert (29) using cytidylic acid incorporation into the total viscera of rat. These investigators isolated the base and sugar portions of the incorporated deoxycytidine and found that each component had been incorporated to essentially the same extent. The previously mentioned experiments were performed entirely in vivo and on heterogenous preparations (total viscera) or on abnormal tissues (regenerating liver or neoplastic tissue). The purpose of this thesis was to investigate the possible existence of an alternate pathway of incorporation using a single tissue. Thus, the in vitro and in vivo incorporation of c14 cytidylic acid into rat kidney was studied.

Comments

A Thesis submitted to the Faculty of the Graduate School, Marquette University in Partial Fulfillment of the Requirements for the Degree of Master of Science, Milwaukee, Wisconsin.

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