Mikeworth et al. 2023 Data Archive Index

Figure 1

- Mikeworth_2023_Figure1_Graphs_STATS.pzfx
- Graphpad Prism file with data for the graphs and statistics presented in Figure 1
- Mikeworth_2023_RawData_Figure1_xlsx
- Excel spreadsheet of all the raw data used to build Figure 1

Figure 2

- Mikeworth_2023_Figure2_Graphs_STATS.pzfx
- Graphpad Prism file with data for the graphs and statistics presented in Figure 2
- Mikeworth_2023_Figure2_RawData.xlsx
- Excel spreadsheet of all the raw data used to build Figure 2

Figure 3

- Mikeworth_2023_Figure3A_Graphs_STATS.pzfx
- Graphpad Prism file with data for the graphs and statistics presented in Figure 3A
- Mikeworth_2023_Figure3A_RawData.xlsx
- Excel spreadsheet of all the raw data used to build Figure 3A
- Mikeworth_2023_Figure3B_Graphs_GRAPH.pzfx
- Graphpad Prism file with data for the graphs presented in Figure 3B
- Mikeworth_2023_Figure3B_STATS_GRAPH.pzfx
- Graphpad Prism file with data for the statistics presented in Figure 3B
- Mikeworth_2023_Figure3B_RawData.xlsx
- Excel spreadsheet of all the raw data used to build Figure3B

Figure 4

- Images used Figure 4
- All images in both jpg and tiff format that were used to build Figure 4
- Mikeworth_2023_Figure4_Graphs_STATS.pzfx
- Graphpad Prism file with data for the graphs and statistics presented in Figure 4
- Mikeworth_2023_Figure4_RawData.xlsx
- Excel spreadsheet of all the raw data used to build Figure 4
- DIC Images Scored
- Files of Images used to score sperm localization
- Each subfolder is labeled by specific strain and temperature
- Within each folder Images are organized by data
- For each image there is a separate DIC, mitotracker and Merged channels file

Figure 5

- Images used in Figure 5
- Images used to build Figure 5
- Mikeworth_2023_Figure5_Graphs.pzfx
- Graphpad Prism file with data for the graph presented in Figure 5
- Mikeworth_2023_Figure5_RawData.xlsx
- Excel spreadsheet of all the raw data used to build Figure 5
- BoDipy Images Scored
- In the BoDipy Images scored folder there are separate folders for each specific strain and temperature
- Within each folder Images are organized by data
- Each image is a DIC image of the proximal region of a single germline arm from an individual hermaphrodite
- This experiment also stained using BoDipy but those data are not contained in this publication and that channel of the image is not included
- DIC Images Scored
- In the DIC images scored folder there are separate folders for each specific strain and temperature
- Within each folder Images are organized by data
- Within each date there are folders for each worm images
- Each worm folder contains a series of DIC images that tile along the entire length of the worm being imaged
- In some cases the folder also contains a composite of all of the DIC images forming an image of the complete worm

Figure S1

- lnp0043 l1 20 pgl-1 568 10k gfplin35 60x 1x 5 11 22 15-maxip.tif (RGB).tif
- Maximum projection of merged channel image of DAPI and LIN-35::GFP from strain of an L1 animal at 20C from LNP0043 used to make Figure S1
- lnp0043_20c_agfp_1_100_60x_germline3_033023-maxip(RGB)_DAPIGFP_levels_300dpi.tif
- Maximum projection of merged channel image of DAPI and LIN-35::GFP from strain of an Adult animal at 20C from LNP0043 used to make Figure S1

Figure S2

Raw uncut image of gels used in Figure S2