A Spinal Projection of 5-Hydroxytryptamine Neurons in the Lamprey Brainstem; Evidence from Combined Retrograde Tracing and Immunohistochemistry

Authors

Lennart Brodin, Karolinska Institute
James T. Buchanan, Marquette UniversityFollow
T. Hökfelt, Karolinska InstituteFollow
Stan Grillner, Karolinska InstituteFollow
A.A.J. Verhofstad, University of NijmegenFollow

Document Type

Article

Language

eng

Format of Original

5 p.

Publication Date

6-6-1986

Publisher

Elsevier

Source Publication

Neuroscience

Source ISSN

0304-3940

Abstract

To investigate whether there is a descending contribution to the spinal 5-hydroxytryptamine (5-HT) innervation in the lamprey, a primitive vertebrate, a study using retrograde transport of the fluorescent tracer Fast blue combined with 5-HT immunohistochemistry was conducted. Two to 4 weeks after an injection of Fast blue into the rostral spinal cord, retrogradely labelled cells were seen throughout the brainstem. Two groups of these cells, one within the posterior reticular nucleus of the rhombencephalon and another rostral to the trigeminal motor nucleus, were labelled after incubation with 5-HT antiserum. These findings suggest that in addition to the well-described local intraspinal 5-HT system, there is also, as in higher vertebrates, a descending 5-HT projection from the brainstem which extends at least 20 segments into the spinal cord.

Comments

Neuroscience Letters, Vol. 67, No. 1 (June 6, 1986): 53-57. DOI.

James Buchanan was affiliated with the Karolinska Institute at the time of publication.

Recommended Citation

Brodin, Lennart; Buchanan, James T.; Hökfelt, T.; Grillner, Stan; and Verhofstad, A.A.J., "A Spinal Projection of 5-Hydroxytryptamine Neurons in the Lamprey Brainstem; Evidence from Combined Retrograde Tracing and Immunohistochemistry" (1986). Biological Sciences Faculty Research and Publications. 222.
https://epublications.marquette.edu/bio_fac/222