XX/XY Sex Chromosomes in the South American Dwarf Gecko (Gonatodes humeralis)

Authors

Tony Gamble, Marquette UniversityFollow
Erin McKenna, Castleton University
Wyatt Meyer, Marquette University
Stuart V. Nielsen, Marquette UniversityFollow
Brendan J. Pinto, Marquette UniversityFollow
Daniel P. Scantlebury, University of RochesterFollow
Timothy E. Higham, University of California - Riverside

Document Type

Article

Language

Eng

Publication Date

6-2018

Publisher

Oxford University Press

Source Publication

Journal of Heredity

Source ISSN

0022-1503

Abstract

Sex-specific genetic markers identified using restriction site-associated DNA sequencing, or RADseq, permits the recognition of a species’ sex chromosome system in cases where standard cytogenetic methods fail. Thus, species with male-specific RAD markers have an XX/XY sex chromosome system (male heterogamety) while species with female-specific RAD markers have a ZZ/ZW sex chromosome (female heterogamety). Here, we use RADseq data from 5 male and 5 female South American dwarf geckos (Gonatodes humeralis) to identify an XX/XY sex chromosome system. This is the first confidently known sex chromosome system in a Gonatodes species. We used a low-coverage de novo G. humeralis genome assembly to design PCR primers to validate the male-specificity of a subset of the sex-specific RADseq markers and describe how even modest genome assemblies can facilitate the design of sex-specific PCR primers in species with diverse sex chromosome systems.

Comments

Accepted version. Journal of Heredity, Vol. 109, No. 4 (June 2018): 462-468. DOI. © 2018 The American Genetic Association. Used with permission.

Recommended Citation

Gamble, Tony; McKenna, Erin; Meyer, Wyatt; Nielsen, Stuart V.; Pinto, Brendan J.; Scantlebury, Daniel P.; and Higham, Timothy E., "XX/XY Sex Chromosomes in the South American Dwarf Gecko (Gonatodes humeralis)" (2018). Biological Sciences Faculty Research and Publications. 608.
https://epublications.marquette.edu/bio_fac/608