Oxford University Press
Journal of Heredity
Sex-specific genetic markers identified using restriction site-associated DNA sequencing, or RADseq, permits the recognition of a species’ sex chromosome system in cases where standard cytogenetic methods fail. Thus, species with male-specific RAD markers have an XX/XY sex chromosome system (male heterogamety) while species with female-specific RAD markers have a ZZ/ZW sex chromosome (female heterogamety). Here, we use RADseq data from 5 male and 5 female South American dwarf geckos (Gonatodes humeralis) to identify an XX/XY sex chromosome system. This is the first confidently known sex chromosome system in a Gonatodes species. We used a low-coverage de novo G. humeralis genome assembly to design PCR primers to validate the male-specificity of a subset of the sex-specific RADseq markers and describe how even modest genome assemblies can facilitate the design of sex-specific PCR primers in species with diverse sex chromosome systems.
Gamble, Tony; McKenna, Erin; Meyer, Wyatt; Nielsen, Stuart V.; Pinto, Brendan J.; Scantlebury, Daniel P.; and Higham, Timothy E., "XX/XY Sex Chromosomes in the South American Dwarf Gecko (Gonatodes humeralis)" (2018). Biological Sciences Faculty Research and Publications. 608.
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